Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
LHX2

Cell type

Cell type Class
Pluripotent stem cell
Cell type
hESC derived retinal cells
NA
NA

Attributes by original data submitter

Sample

source_name
hES-RPE
cell type
ES-derived RPE cells
chip antibody
rabbit anti-Lhx2 (Millipore, ABE1402)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Sonicated chromatin from hES-RPE cells was immunoprecipitated with rabbit anti-Lhx2 (Millipore, ABE1402) or gout anti-Lhx2 (Santa Cruz, sc-19344) Libraries were prepared according to Illumina's instructions accompanying the DNA Sample Prep Kit.

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
22310719
Reads aligned (%)
97.5
Duplicates removed (%)
21.2
Number of peaks
923 (qval < 1E-05)

hg19

Number of total reads
22310719
Reads aligned (%)
96.4
Duplicates removed (%)
22.5
Number of peaks
864 (qval < 1E-05)

Base call quality data from DBCLS SRA